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用途
sufficient for 100 colorimetric or fluorometric tests
输入
culture(s)
plasma
tissue
serum
urine
检测方法
colorimetric
fluorometric
相关疾病
cancer
储存温度
−20°C
一般描述
Citrate is an intermediate in the citric acid cycle and is involved in fatty acid synthesis. The Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample. Linear detection range: 4 to 400 μM citrate for colorimetric assays and 0.5 to 40 μM for fluorimetric assays.
应用
The Citrate Assay Kit may be used for:
- Cancer Research
- Drug Discovery
- Metabolic Disorders Research
特点和优势
Efficient and Precise: Detect Citrate levels ranging from 4 to 400 μM (colorimetric) and 0.5 to 40 μM (fluorimetric) in a 15 minute room temperature reaction, providing both convenience and sensitivity.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
适用性
Suitable for citrate determination in biological samples (e.g. plasma, serum, urine, tissue and culture media.)
原理
In this kit, citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample. Linear detection range: 4 to 400 μM citrate for colorimetric assays and 0.5 to 40 μM for fluorimetric assays.
其他说明
For additional information on our range of Biochemicals, please complete this form.
储存分类代码
10 - Combustible liquids
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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