Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
About This Item
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质量水平
方案
≥99.5% (titration)
表单
crystalline powder
储存条件
dry at room temperature
颜色
white
有效pH范围
5.5-6.7
pKa
6.1
溶解性
water: 0.25 g/mL, clear, colorless
应用
agriculture
clinical research
diagnostic assay manufacturing
life science and biopharma
储存温度
room temp
SMILES字符串
O.OS(=O)(=O)CCN1CCOCC1
InChI
1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2
InChI key
MIIIXQJBDGSIKL-UHFFFAOYSA-N
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一般描述
MES缓冲液具有良好的水溶性并且不溶于脂质，它不能够渗透细胞膜，这一点确保了它在细胞环境中的功效。它的应用包括维持细胞培养基、蛋白质基缓冲液和电泳缓冲液的稳定性。MES缓冲液可用于纯化抗体、肽、蛋白质、血液成分和生长因子。MES 缓冲液可作为甲次砷酸盐和非两性离子缓冲液（如柠檬酸盐和苹果酸盐）等有毒试剂的更良好的替代品。在各种实验环境中，MES 缓冲液可用于调节生长培养基的pH值，稳定酶溶液，并可作为PAGE电泳缓冲液的成分。
应用
特点和优势
- 在pH 5.5-6.7 范围内可有效缓冲，pKa 值为 6.1 (25 °C)
- 易溶于水
- 金属离子结合能力极低
- 在宽pH范围内保持稳定
- 低紫外吸收率和极低的反应性
制备说明
储存及稳定性
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves
历史批次信息供参考:
分析证书(COA)
其他客户在看
实验方案
在测定变溶菌素活性时，使用了一种在600 nm下的连续分光光度法进行测定。粪链球菌细胞壁被用作酶的底物。
This procedure may be used for Mutanolysin products.
Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)
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Discover high-purity biological buffers for production scale. Ensure stability, pH control, and consistent supply with our expert manufacturing solutions.
了解生产规模的高纯度生物学缓冲液通过我们专业制造溶液，确保稳定性、pH控制和稳定供应。
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Helpful?
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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