H0393

[Lys⁴⁷]Hirudin (HV2), leech

recombinant, expressed in unspecified host, ≥7,000 ATU/mg protein, powder

• CAS号: 8001-27-2
• EC號碼: 232-279-1
• MDL號碼: MFCD00145679
• 分類程式碼代碼: 12352202
• NACRES: NA.77

属性

• product name: [Lys⁴⁷]Hirudin (HV2), leech, recombinant, expressed in unspecified host, ≥7,000 ATU/mg protein (ATU = antithrombin units)
• 重組細胞: expressed in unspecified host
• 品質等級: 200
• 形狀: powder
• 比活性: ≥7,000 ATU/mg protein (ATU = antithrombin units)
• 分子量: ~7 kDa
• 溶解度: pyridine: soluble; water: soluble
• 運輸包裝: dry ice
• 儲存溫度: −20°C

说明

應用

Antithrombotic efficacy by parenteral route is substantially prolonged by incorporation into cationic liposomes, which act as a sort of sustained-release device.

物理性質

The anticoagulant hirudin is a ~7 kDa acidic protein containing 65 amino acid residues. It occurs naturally in leeches (Hirudo medicinalis). It is the most potent natural inhibitor of both soluble and clot-bound thrombin. Hirudin binds thrombin with high affinity and covers more than 20% of the surface area of thrombin, occluding both the active site and exosite I (fibrinogen and PAR recognition site). This coverage blocks thrombus growth and platelet activation. Hirudin is not metabolized in the bloodstream of humans and is eliminated unchanged via kidney filtration. Native hirudin contains a sulfated tyrosyl residue (Tyr63), three disulfide bridges, and a high proportion of dicarboxylic acids. Hirudin is not glycosylated, and lacks tryptophan, arginine, and methionine residues. At least 20 isoforms have been identified and sequenced.

This recombinant hirudin product corresponds to the HV-1 variant sequence, with the exception that the Tyr⁶³ residue is not sulfated.

單位定義

One unit (ATU) will neutralize one NIH unit of thrombin at 37 °C, based on direct comparison to an NIH thrombin reference standard.

重構

Hirudin is soluble in water. The literature cites the use of "Dilution Fluid II" (35.7 mM acetic acid, 35.7 mM sodium diethyl barbiturate, 0.85% NaCl, 1% bovine serum albumin, and 0.5% PEG, pH 7.4) to dissolve hirudin (500 ATU/mL) and thrombin. Hirudin is reported to be soluble in pyridine, but practically insoluble in alcohol, ether, acetone, or benzene.

分析報告

Protein determined by Lowry.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: dust mask type N95 (US), Eyeshields, Gloves