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Merck

T3068

Sigma-Aldrich

凝血酶产生显色底物

≥90% (HPLC), solid

别名:

β-Ala-Gly-Arg-p-硝基苯胺二乙酸酯

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About This Item

经验公式(希尔记法):
C21H34N8O9
分子量:
542.54
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.32

生物源

synthetic (organic)

品質等級

化驗

≥90% (HPLC)

形狀

solid

技術

activity assay: suitable

儲存溫度

2-8°C

Amino Acid Sequence

Ala-Gly-Arg-pNA

應用

凝血酶产生的显色底物可用于通过适体亲和捕获而产生的显色检测,并通过随后的酶反应用于检测稀释的人血清中的凝血酶。

生化/生理作用

凝血酶产生的显色底物也被称为β-Ala-Gly-Arg对硝基苯胺,可被人凝血酶蛋白水解切割并产生β-Ala-Gly-Arg和对硝基苯胺。 对硝基苯胺的释放进行定量后可评估抗凝血酶的活性。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Jan Van Den Abbeele et al.
PLoS pathogens, 6(6), e1000926-e1000926 (2010-06-10)
Tsetse flies are the notorious transmitters of African trypanosomiasis, a disease caused by the Trypanosoma parasite that affects humans and livestock on the African continent. Metacyclic infection rates in natural tsetse populations with Trypanosoma brucei, including the two human-pathogenic subspecies
Qiang Zhao et al.
Biosensors & bioelectronics, 34(1), 232-237 (2012-03-06)
A simple chromogenic assay for human alpha thrombin is developed through aptamer affinity capture and a subsequent enzyme reaction. Thrombin is captured on the aptamer-modified magnetic beads, and catalyzes the conversion of chromogenic substrates to optically measured products. The measurement
D Prasa et al.
Thrombosis and haemostasis, 78(4), 1215-1220 (1997-11-19)
A series of inhibitors of factor Xa (FXa) were investigated using the thrombin generation assay to evaluate the potency and specificity needed to efficiently block thrombin generation in activated human plasma. By inhibiting FXa the generation of thrombin in plasma
Jingjing Wang et al.
Frontiers in microbiology, 12, 778309-778309 (2021-12-21)
African swine fever virus (ASFV) is a large double-stranded DNA virus and causes high mortality in swine. ASFV can be transmitted by biological vectors, including soft ticks in genus Ornithodoros but not hard ticks. However, the underlying mechanisms evolved in
Ning Luan et al.
Toxins, 11(12) (2019-12-11)
Elastase is a globular glycoprotein and belongs to the chymotrypsin family. It is involved in several inflammatory cascades on the basis of cleaving the important connective tissue protein elastin, and is strictly regulated to a balance by several endogenous inhibitors.

实验方案

Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

凝血酶是一种内溶性丝氨酸蛋白酶,可选择性裂解纤维蛋白原的精氨酸-甘氨酸键,从而形成纤维蛋白并释放纤维蛋白肽A和B。

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