推荐产品
包裝
pack of 1 kit
製造商/商標名
Cytiva 28-9321-71
儲存條件
(0.15% Kathon CG)
(20% Ethanol)
基質
highly cross-linked 6% agarose
樣品平均尺寸
34 μm
cleaning
2-14(Ni2+-stripped medium.)
工作範圍
3-12(Ni2+-stripped medium.)
容量
≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)
儲存溫度
2-8°C
一般說明
特點和優勢
- For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
- Ni Sepharose High Performance in prepacked spin columns.
- Yields up to 750 µg pure protein per column.
- Allows direct purification of both clarified and unclarified cell lysates.
成分
1 pack - GE28-4013-53 - His Spin Trap™
1 pack - GE11-0034-00 - His Buffer Kit
儲存和穩定性
分析報告
法律資訊
试剂盒组分也可单独购买
- GE11-0034-00His Buffer Kit, Cytiva 11-0034-00化学品安全说明书
- GE28-4013-53His Spintrap™, Cytiva 28-4013-53化学品安全说明书
訊號詞
Danger
危險聲明
儲存類別代碼
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
其他客户在看
商品
How to optimize purification of histidine-tagged proteins using Cytiva products.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
实验方案
This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from Cytiva.
Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.
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