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Key Documents

GE28-9321-71

HIS Spintrap Kit

Cytiva 28-9321-71, pack of 1 kit

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About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56

包裝

pack of 1 kit

製造商/商標名

Cytiva 28-9321-71

儲存條件

(0.15% Kathon CG)
(20% Ethanol)

基質

highly cross-linked 6% agarose

樣品平均尺寸

34 μm

cleaning

2-14(Ni2+-stripped medium.)

工作範圍

3-12(Ni2+-stripped medium.)

容量

≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

儲存溫度

2-8°C

一般說明

His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

特點和優勢

  • For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
  • Ni Sepharose High Performance in prepacked spin columns.
  • Yields up to 750 µg pure protein per column.
  • Allows direct purification of both clarified and unclarified cell lysates.

成分

The kit contains:

1 pack - GE28-4013-53 - His Spin Trap
1 pack - GE11-0034-00 - His Buffer Kit

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

SpinTrap is a trademark of Cytiva

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • GE11-0034-00His Buffer Kit, Cytiva 11-0034-00化学品安全说明书

  • GE28-4013-53His Spintrap, Cytiva 28-4013-53化学品安全说明书

象形圖

Health hazard

訊號詞

Danger

危險聲明

儲存類別代碼

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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商品

How to optimize purification of histidine-tagged proteins using Cytiva products.

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

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实验方案

This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from Cytiva.

Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.

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