GE17-5319-01

HisTrap^™ Fast Flow

Cytiva 17-5319-01, pack of 5 × 1 mL

• UNSPSC代码: 23151816
• NACRES: NA.56

属性

• 包装: pack of 5 × 1 mL
• 制造商/商品名称: Cytiva 17-5319-01
• 参数: <20 mL/min flow rate; 42 psi
• 柱床尺寸: 16 mm × 25 mm
• 柱床体积: 5 mL
• 色谱柱内径: 16 mm
• 基质: 6% cross-linked agarose
• 粒径: 45-165 μm
• 平均直径: 90 μm
• cleaning: 2-14(Ni²⁺-stripped medium.)
• 工作范围: 3-12(Ni²⁺-stripped medium.)
• 容量: ~40 mg binding capacity(histidine-tagged protein)
• 适用性: suitable for bioprocess medium

说明

一般描述

HisTrap^™ FF is a ready-to-use column, prepacked with precharged Ni Sepharose^™ 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).

Ni Sepharose^™ 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni²⁺ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni²⁺ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

The high flow rates made possible by the Ni Sepharose^™ 6 Fast Flow matrix make HisTrap^™ FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap^® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.

特点和优势

• High binding capacity, approx. 40 mg/mL medium.
• Negligible leakage of Ni²⁺.
• Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

HiTrap is a registered trademark of Cytiva

HisTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

安全信息

• 象形图: GHS09
• 警示用语：: Warning
• 危险声明: H226
• 预防措施声明: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 储存分类代码: 3 - Flammable liquids