GE28-4017-45

GSTrap^™ 4B

Cytiva 28-4017-45, pack of 5 × 1 mL

• UNSPSC代码: 23151816
• NACRES: NA.56

属性

• ligand: glutathione
• 表单: prepacked column
• 交联: cross-linked
• 包装: pack of 5 × 1 mL
• 制造商/商品名称: Cytiva 28-4017-45
• 储存条件: (20% Ethanol)
• 参数: <4 mL/min flow rate; 42 psi (H₂O at 25°C)
• 技术: liquid chromatography (LC): suitable
• 柱床尺寸: 7 mm × 25 mm
• 柱床体积: 1 mL
• 色谱柱内径: 7 mm
• 基质: 4% agarose
• 基质活性基团: glutathione functional group
• 粒径: 45-165 μm
• 平均直径: 90 μm
• cleaning: 4-13
• 工作范围: 4-13
• 容量: >25 mg binding capacity (horse liver GST/ml medium)
• 分离技术: affinity

说明

一般描述

GSTrap^™ 4B columns are prepacked Glutathione Sepharose^™ 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

应用

GST, see the section for licensing information in our terms and conditions, tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap^™ 4B. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose^™ 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA^® design or FPLC^™ System.

特点和优势

• High binding capacity.
• Mild elution conditions preserving Protein antigenicity and function.
• Easy one-step purifications of Glutathione S-Transferase (GST) tagged Proteins resulting in high purity.
• GSTrap^™ 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTA^® design.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他说明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律信息

FPLC is a trademark of Cytiva

GSTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

安全信息

• 象形图: GHS02
• 警示用语：: Warning
• 危险声明: H226
• 预防措施声明: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 储存分类代码: 3 - Flammable liquids