推荐产品
物料
resin (Sephadex G-25 DNA grade F)
无菌性
non-sterile
制造商/商品名称
Cytiva 27-5325-01
包装
pkg of 50 columns
工作体积
10 —100 μL
粒径
20-80 μm (dry)
孔径
5000 Da MWCO
工作范围
2-13
相关类别
一般描述
MicroSpin™ G-25 columns can be used for any DNA greater than 10 bases in length and are therefore an excellent choice for the purification of oligonucleotides or very small DNA fragments following synthesis or a labelling reaction. They will not remove or denature enzyme.
特点和优势
- For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 μl using spin-column chromatography.
- Excellent for rapid purification of newly synthesized oligonucleotides >10-mers in 100 to 150 μl of deprotection solution using spin-column chromatography.
- Convenient: Prepacked with Sephadex® G-25 DNA Grade and pre-equilibrated in distilled water containing 0.05% Kathon CG/ICP Biocide.
- Ready to use: Requires less than 4 min from sample application to collection of purified product.
- Tested in nickase, single and double-stranded exonuclease and RNase assays.
- Can also be used for desalting/buffer exchange of DNA and removal of unincorporated radionucleotides from end-labeled oligonucleotides (at least 10 bases in length) in a volume of 10 to 100 μl.
储存及稳定性
分析说明
法律信息
储存分类代码
12 - Non Combustible Liquids
其他客户在看
商品
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
实验方案
DNA cleanup using illustra™ GFX kits from Cytiva.
This page shows how to condition membrane proteins for further analysis with products from Cytiva.
如何使用Sephadex G-25、HiTrap脱盐柱或ÄKTAprime plus进行缓冲液置换和脱盐。
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
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