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ligand
quaternary amine
描述
Ion Exchanger Type (value)
包裝
pkg of 7 ea
製造商/商標名
Cytiva 17-6002-33
參數
42 psi
床尺寸
7 mm × 25 mm
床體積
1 mL
柱內徑
7 mm
基質
6% cross-linked agarose
粒徑
45-165 μm
平均直徑
90 μm
cleaning
2-14
工作範圍
2-12
適合性
suitable for bioprocess medium
一般說明
Q, SP, DEAE, and CM Sepharose™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.Q and SP Sepharose™ XL media have long chains of dextran coupled to a robust, 6% highly cross-linked agarose matrix. The dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities. The active end of the charged group is the same for DEAE Sepharose Fast Flow and ANX Sepharose™ Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose™ 4 Fast Flow (high sub) has a diethylaminopropyl group attached. After choosing the optimal medium, prepacked columns and bulk media are available for larger scale preparative work.
特點和優勢
- Fast and easy selection with seven different ion-exchange ligands on Sepharose™ Fast Flow and Sepharose™ XL, prepacked in 1 mL HiTrap® columns.
- Offers a fast, easy and convenient way screen for the optimal media for a given application .
- Excellent for optimization studies; buffer composition, pH, flow rate, sample loading, and elution scheme can be optimized with small sample quantities prior to scale-up.
- Designed for use with a syringe, peristaltic pump, or chromatography system such as AKTA design.
儲存和穩定性
分析報告
法律資訊
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
其他客户在看
商品
This page provides information about the principles and standard conditions for different purification techniques for the purification of multiprotein complexes with from Cytiva.
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
实验方案
This page covers the principles and methods of chromatofocusing, a chromatography technique that separates proteins according to differences in their isoelectric point (pI).
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
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