GE17-5130-02

GSTrap^™ Fast Flow

Cytiva 17-5130-02, pack of 2 × 1 mL

• UNSPSC代码: 23151817
• NACRES: NA.25

属性

• ligand: glutathione and 10-carbon linker arm
• 包装: pack of 2 × 1 mL
• 制造商/商品名称: Cytiva 17-5130-02
• 参数: <4 mL/min flow rate; 42 psi
• 柱床尺寸: 7 mm × 25 mm
• 柱床体积: 1 mL
• 色谱柱内径: 7 mm
• 基质: highly cross-linked 4% agarose
• 平均直径: 90 μm
• cleaning: 3-12
• 工作范围: 3-12
• 容量: >10 mg/mL binding capacity

说明

一般描述

GSTrap^™ FF columns are prepacked Glutathione Sepharose^™ Fast FLow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

应用

GST*-tagged Proteins can be purified directly from pretreated bacterial lysates using GSTrapTM FF.GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged Proteins can be detected using colorimetric or immunological methods. The medium, Glutathione SepharoseTM 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTATM design or FPLCTM System.

特点和优势

• Fast and simple one-step purification of GST-tagged Proteins.
• Mild elution conditions preserving Protein antigenicity and function.
• Easy scale-up by connecting the columns in series.
• Sepharose^™ 4 Fast FLow provides good fLow properties.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他说明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律信息

GSTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

安全信息

• 象形图: GHS02
• 警示用语：: Warning
• 危险声明: H226
• 预防措施声明: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 储存分类代码: 3 - Flammable liquids