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Merck

GE17-0110-01

Sepharose 6B

Cytiva 17-0110-01, pack of 1 L

别名:

珠状琼脂糖

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About This Item

CAS号:
MDL编号:
UNSPSC代码:
41106500
NACRES:
NA.56
价格与库存信息目前不能提供

特点

autoclavable Autoclavable, 20 min at 120°C in pH 7

包装

pack of 1 L

制造商/商品名称

Cytiva 17-0110-01

基质

6% agarose

粒径

45-165 μm

cleaning in place

4-9

工作范围

4-9

SMILES字符串

OC[C@H]1O[C@@H](O[C@@H]2[C@@H]3CO[C@H]2[C@H](O)[C@@H](O3)O[C@H]4[C@@H](O)[C@@H](CO)O[C@@H](O[C@@H]5[C@@H]6CO[C@H]5[C@H](O)[C@H](O)O6)[C@@H]4O)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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一般描述

Sepharose 6B is a well-proven agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

Sepharose is a bead-formed agarose-based gel filtration matrix. Sepharose is available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose 2B, Sepharose 4B and Sepharose 6B respectively, Both Sepharose and Sepharose CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight.

Sepharose CL gels are cross-linked derivatives of Sepharose 2B, Sepharose 4B and Sepharose 6B. The cross-linked form of Sepharose is chemically and physically more resistant than Sepharose itself, offering the same selectivity with better flow characteristics. Cross-linked Sepharose gels are resistant to organic solvents and are thus the choice for separations in organic solvents.

特点和优势

  • 6% Agarose gel filtration media.
  • Proven base matrix for coupling affinity ligands

其他说明

珠状琼脂糖用于分离高分子量的分子。交联珠状琼脂糖更能耐受变性条件,从而为样本缓冲液和洗脱液的选择提供更多的灵活性。近似的 % 琼脂糖浓度由产品名 (Ultrogel A%) 表示。

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

Sepharose is a trademark of Cytiva

象形图

Flame

警示用语:

Warning

危险声明

储存分类代码

3 - Flammable liquids


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Purification of recombinant protein derived from the baculovirus expression system using glutathione affinity agarose.
E J Sorscher et al.
Methods in molecular biology (Clifton, N.J.), 39, 337-348 (1995-01-01)
J Porath
Journal of molecular recognition : JMR, 3(3), 123-127 (1990-06-01)
Protein adsorption and retention data collected from recent chromatographic studies on hydrophilic gels substituted with chelate-bonded metal ions are discussed. Attempts are made to interpret the adsorption behavior in terms of molecular events caused by the affinity for the immobilized
G Duro et al.
Journal of chromatography, 618(1-2), 95-104 (1993-08-25)
Agarose gel electrophoresis is a powerful technique for the separation of nucleic acids on the basis of their size and conformation. The development of methods to recover size-fractionated nucleic acids molecules from agarose gels has greatly facilitated recombinant DNA technologies.
Ian G Cowell et al.
Mutagenesis, 26(2), 253-260 (2010-11-12)
The ability to detect and quantify specific DNA adducts benefits genome stability research, drug development and the evaluation of environmental mutagens. The trapped in agarose DNA immunostaining (TARDIS) assay was developed as a means of detecting and quantifying melphalan and
F H Kirkpatrick et al.
Electrophoresis, 14(4), 349-354 (1993-04-01)
Properties of agarose potentially relevant to PFGE (pulsed-field gel electrophoresis) are reviewed, and some new information is presented. Agarose polymers appear to have molecular weights in the range of 100,000 to 200,000 Da, but this is not tightly related to

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