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生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
whole antiserum
抗体产品类型
primary antibodies
克隆
polyclonal
包含
15 mM sodium azide
种属反应性
human
技术
dot blot: 1:50 using galactocerebroside I and II
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... GALC(2581)
一般描述
Galactocerebroside is the major galactosphingolipid of myelin. It is expressed in the CNS in cell membranes of oligodendrocytes. It is also expressed in Schwann cells in peripheral nerves.
This product is a pooled delipidized antiserum produced in rabbits by repeated injections of a mixture of galactocerebrosides I and II conjugated to keyhole limpet hemocyanin.
特异性
The antibody localizes oligodendroglia in cultured cells. It may be used for studying changes in the organization of oligodendroglial membrane sheets in culture. Specificity of the product is determined by dot blotting using galactocerebroside as the antigen.
免疫原
Galactocerebrosides (GalC) I and II conjugated to KLH
应用
Anti-Galactocerebroside antibody produced in rabbit has been used:
- for dot-blot immunoassay and western blot analysis of mouse brain homogenates
- as a primary antibody at a working dilution of 1:50 for immunostaining of muscle tissue of Rana pipiens frogs
- as a primary antibody at a working dilution of 1:500 in immunofluorescence and immunohistochemistry using primary human astroglia
生化/生理作用
Galactocerebroside is the major galactosphingolipid of myelin. It is expressed in the CNS in cell membranes of oligodendrocytes. It is also expressed in Schwann cells in peripheral nerves. Galactocerebroside is found in serum and cerebral spinal fluid of patients with demyelinating diseases like multiple sclerosis.
Galactocerebroside was shown to play an important role in the processes of normal development and myelination and is known to be an early marker of oligodendrocyte development.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
C A Dyer et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 8(11), 4307-4318 (1988-11-01)
Antibodies to galactocerebroside (GalC) cause major changes in the organization of the membrane sheets elaborated by murine oligodendroglia in culture. Exposure of oligodendroglia to rabbit anti-GalC IgG for 15 min followed by fluoresceinated second antibodies results in patches of surface
D M Kokkinakis et al.
Neuro-oncology, 3(2), 99-112 (2001-04-12)
Glial tumors may originate from the malignant transformation of multipotent glial progenitor cells, but tools to study malignant transformation leading to gliomas are limited by the lack of biological systems that represent early stages of this disease in adult animals.
J W Prineas et al.
Laboratory investigation; a journal of technical methods and pathology, 61(5), 489-503 (1989-11-01)
Fresh lesions in the brain and spinal cord of patients with multiple sclerosis who died shortly after the onset of symptoms were examined immunocytochemically for myelin and oligodendrocyte antigens that are known to be sequentially expressed during normal development. Cells
Lead alters the developmental profile of the galactolipid metabolic enzymes in cultured oligodendrocyte lineage cells
Deng W and Poretz RD
Neurotoxicology, 22(4), 429-437 (2001)
J Georgiou et al.
Glia, 27(2), 101-109 (1999-07-27)
Perisynaptic Schwann cells (PSCs) envelop axonal terminals and are physiologically distinct from the nearby myelinating Schwann cells (MSCs), which surround the same innervating motor axons. PSCs have special functions at the neuromuscular synapse, where they detect and can modulate neurotransmitter
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