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重組細胞
expressed in E. coli
品質等級
形狀
lyophilized powder
比活性
≥550 units/mg protein (biuret)
成份
Protein, 10-40% biuret
應用
agriculture
異物活動
creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01%
儲存溫度
2-8°C
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相关类别
應用
肠系膜明串珠菌来源葡萄糖 6-磷酸脱氢酶已用于：
- PGI 的偶联分光光度测定法（F6P-G6P 反应）
- 使用烟草叶研究淀粉时程
- 测定北美黑橡(Quercus velutina Lam. saplings)根和茎样品中的葡萄糖含量
- 使用野生型和转基因拟南芥整株幼苗的淀粉样本测定葡萄糖
生化／生理作用
6-磷酸葡萄糖脱氢酶 (G6PD) 在所有组织中均有表达，并产生还原形式的烟酰胺腺嘌呤二核苷酸磷酸 (NADPH)，有助于细胞避免氧化压力。G6PD不足是一种 X 连锁的遗传性基因缺陷，由G6PD基因常见突变引起。
葡萄糖-6-磷酸脱氢酶(G6PD)催化葡萄糖-6-磷酸转化为6-磷酸葡糖酸内酯，作为磷酸戊糖途径的第一步。
單位定義
在 NAD 存在下，在pH 7.8，30°C 下，1 个单位将每分钟氧化 1.0 μmol D-6-磷酸葡萄糖至 6-磷酸-D-葡萄糖酸盐。
外觀
含有Ficoll和Tris 缓冲盐的冻干粉
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
dust mask type N95 (US), Eyeshields, Gloves
其他客户在看
Alyssa L Preiser et al.
Frontiers in plant science, 11, 580726-580726 (2020-12-29)
Phosphoglucoisomerase (PGI) isomerizes fructose 6-phosphate (F6P) and glucose 6-phosphate (G6P) in starch and sucrose biosynthesis. Both plastidic and cytosolic isoforms are found in plant leaves. Using recombinant enzymes and isolated chloroplasts, we have characterized the plastidic and cytosolic isoforms of
Ching Man Wai et al.
PLoS genetics, 15(6), e1008209-e1008209 (2019-06-15)
Plants with facultative crassulacean acid metabolism (CAM) maximize performance through utilizing C3 or C4 photosynthesis under ideal conditions while temporally switching to CAM under water stress (drought). While genome-scale analyses of constitutive CAM plants suggest that time of day networks
Benjamin J Reisman et al.
Nature chemical biology, 18(4), 360-367 (2021-12-04)
Cancer cells have long been recognized to exhibit unique bioenergetic requirements. The apoptolidin family of glycomacrolides are distinguished by their selective cytotoxicity towards oncogene-transformed cells, yet their molecular mechanism remains uncertain. We used photoaffinity analogs of the apoptolidins to identify
Thomas Beneyton et al.
Nature communications, 9(1), 2391-2391 (2018-06-21)
Self-sustained metabolic pathways in microcompartments are the corner-stone for living systems. From a technological viewpoint, such pathways are a mandatory prerequisite for the reliable design of artificial cells functioning out-of-equilibrium. Here we develop a microfluidic platform for the miniaturization and
Shinichi Harada et al.
The Journal of pharmacology and experimental therapeutics, 344(1), 276-285 (2012-11-03)
Orexin-A (a glucose-sensing neuropeptide in the hypothalamus) and brain-derived neurotrophic factor (BDNF; a member of the neurotrophin family) play roles in many physiologic functions, including regulation of glucose metabolism. We previously showed that the development of postischemic glucose intolerance is
实验方案
在测定6-磷酸葡萄糖脱氢酶活性时，使用β-烟酰胺腺嘌呤二核苷酸磷酸在340 nm处通过分光光度法进行测定。
To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.
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