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重組細胞
expressed in baculovirus infected Sf9 cells
品質等級
產品線
PRECISIO® Kinase
化驗
≥70% (SDS-PAGE)
形狀
buffered aqueous glycerol solution
比活性
143-193 nmol/min·mg
分子量
~48 kDa
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... GSK3B(2932)
一般說明
GSK3B（糖原合成酶激酶 3β）是 GSK3 酶的两种亚型之一，两者由不同的基因编码。这种蛋白质主要是细胞质，但也被发现集中在细胞核和线粒体。是一种蛋白质-丝氨酸激酶。该蛋白的核心含有一个蛋白激酶结构域，由 482 个氨基酸组成，分子量为 46712 Da。其组织表达范围广，在脑内表达量最高。
應用
GSK3β, active, His tagged human has been used as an incubating medium during in vitro hyperphosphorylation of tau protein.
生化／生理作用
GSK3β是一种丝氨酸苏氨酸蛋白激酶，最初被确定为磷酸化和抑制糖原合成酶的激酶。GSK3β广泛存在于人体组织中，参与调节多个生理过程，包括通过胰岛素控制糖原和蛋白质合成以及调节转录因子 AP-1 和 CREB。人 GSK3β瞬时转染到稳定转染单个人 tau 蛋白亚型的中国仓鼠卵巢细胞中，在磷酸化依赖性抗 tau 蛋白抗体研究的所有位点均导致 tau 蛋白过度磷酸化。
糖原合成酶激酶 3β（GSK3β）通过在 Thr-286 上将其磷酸化而负责快速的细胞周期蛋白 D1 周转。在胚胎发育和成体组织细胞增殖过程中作为 Wnt 信号的负调节因子发挥作用。这种蛋白质磷酸化和调节多种蛋白质，如糖原合成酶、胰岛素受体底物-1、轴蛋白和 APC（腺瘤性结肠息肉病）。这种蛋白也被证明与多种与阿尔茨海默病 (AD) 相关的蛋白相互作用。
外觀
50 mM 磷酸氢二钠 pH 7.0、300 mm NaCl、150 mm 咪唑、0.1 mM PMSF、0.2 mm DTT、25% 甘油中提供。
法律資訊
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Danger
危險聲明
危險分類
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
Lannah S Abasi et al.
Communications biology, 7(1), 251-251 (2024-03-02)
Tau is a microtubule-associated protein often found in neurofibrillary tangles (NFTs) in the brains of patients with Alzheimer's disease. Beyond this context, mounting evidence suggests that tau localizes into the nucleus, where it may play a role in DNA protection
B R Sperber et al.
Neuroscience letters, 197(2), 149-153 (1995-09-08)
Hyperphosphorylated tau protein is the major constituent of the paired helical filament (PHF), the major fibrous component of the neurofibrillary lesions of Alzheimer's disease (AD). Hyperphosphorylation of tau is believed to be the critical event that leads to filament assembly.
Glycogen synthase kinase 3 in Wnt signaling pathway and cancer
Nydia Tejeda-Mu?oz
IUBMB Life (2015)
C Sutherland et al.
The Biochemical journal, 296 ( Pt 1), 15-19 (1993-11-15)
The beta-isoform of glycogen synthase kinase-3 (GSK3 beta) isolated from rabbit skeletal muscle was inactivated 90-95% following incubation with MgATP and either MAP kinase-activated protein kinase-1 (MAPKAP kinase-1, also termed RSK-2) or p70 S6 kinase (p70S6K), and re-activated with protein
Richard S Jope et al.
Trends in biochemical sciences, 29(2), 95-102 (2004-04-23)
Glycogen synthase kinase-3 (GSK3) is now recognized as a key component of a surprisingly large number of cellular processes and diseases. Several mechanisms play a part in controlling the actions of GSK3, including phosphorylation, protein complex formation, and subcellular distribution.
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