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G3664

Sigma-Aldrich

谷胱甘肽还原酶 来源于面包酵母（酿酒酵母）

ammonium sulfate suspension, 100-300 units/mg protein (biuret)

别名:

GR, NAD(P)H:氧化-谷胱甘肽氧化还原酶

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About This Item

CAS号:
9001-48-3
EC 号:
1.6.4.2 (BRENDA, IUBMB)
MDL编号:
MFCD00131196
UNSPSC代码:
12352204
NACRES:
NA.47
价格与库存信息目前不能提供

表单

ammonium sulfate suspension

质量水平

200

比活

100-300 units/mg protein (biuret)

分子量

118 kDa

UniProt登记号

P41921

异质活性

G-6-PDH, 6-PGDH, and NADPH oxidase ≤0.01%
lipoamide dehydrogenase ≤0.1%

储存温度

2-8°C

基因信息

bakers yeast ... GLR1(856014)

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一般描述

谷胱甘肽还原酶（GLR1）在线粒体和细胞质中存在异构体。它和硫氧还蛋白还原酶有相同的序列，结构同源，是含有黄素的氧化还原酶。它的活性位点是由氧化还原活性二硫组成，催化活性需要NADPH。这种酶广泛存在于植物，细菌，酵母，小鼠和人类。[1]

应用

来自烘焙′s 酵母(Saccharomyces cerevisiae) 的谷胱甘肽还原酶（GR）用于
  • 使用谷胱甘肽还原酶-5,5′-二硫基双（2-硝基苯甲酸）-基酶回收实分析定量心肌组织中的谷胱甘肽含量。[2]
  • 使用略微改良的微谷胱甘肽分析，定量初情期母猪卵母细胞中的还原谷胱甘肽（GSH）[3]
  • 制备总GSSG（谷胱甘肽二硫化物）+GSH检测，然后在大鼠晶状体内所有GSSG被还原成GSH。[4]
  • 在大鼠卵母细胞内定量还原谷胱甘肽（GSH）[5]
来自烘焙′酵母的谷胱甘肽还原酶（GR）用于：
  • 谷胱甘肽分析用来确定谷胱甘肽浓度[3][2]
  • 作为标准产生矫正曲线[6]
  • 作为抗原测量血浆GR活性。[7]

生化/生理作用

谷胱甘肽还原酶 IGR) 是抗氧化系统中关键的黄素酶。谷胱甘肽超氧化物酶使用还原谷胱甘肽（GSH），去除过氧化氢毒性，在这一过程中GSH会被转化成氧化谷胱甘肽（GSSG）。然后GSSG被谷胱甘肽还原酶（GR）回收变成GSH，这一过程中NADPH变成NADP+。 重生的GSH能够解更多的过氧化氢的毒。该酶使用FAD作为辅因子。GR和谷胱甘肽过氧化物酶可能在精子中作为抗氧化酶，抑制脂质过氧化。 谷胱甘肽还原酶和其他一些蛋白质有共同的结构模体，包括天冬氨酸蛋白酶、柠檬酸合成酶、EF手性分子、血红蛋白、脂质体和α/β水解酶。 GR被褪黑素激活，被报道不可逆地被一些氧自由基产生系统抑制。
谷胱甘肽（γ-谷氨酰半胱氨酸甘氨酸）是一种广泛存在的三肽硫醇，在细胞氧化压力抵抗机制中起到关键作用。谷胱甘肽还原酶（GLR1）负责还原谷胱甘肽二硫化物（GSSG），形成还原谷胱甘肽。[1]

单位定义

一单位每分钟能够还原 1.0 μmole氧化谷胱甘肽，pH7.6，25℃。

外形

悬浮于3.6 M (NH4)2SO4, pH 7.0，含0.1 mM二硫苏糖醇

制备说明

通过亲和层析纯化

抑制剂

产品编号
说明
价格

C0400

卡莫司汀, ≥98%

相关产品

产品编号
说明
价格

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000399459
0000399458
0000389538
0000371190
0000371194

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Caryn E Outten et al.
The Journal of biological chemistry, 279(9), 7785-7791 (2003-12-16)
To combat oxidative damage, eukaryotic cells have evolved with numerous anti-oxidant factors that are often distributed between cytosolic and mitochondrial pools. Glutathione reductase, which regenerates the reduced form of glutathione, represents one such anti-oxidant factor, yet nothing is known regarding
Kristin K Brown et al.
Free radical biology & medicine, 45(4), 494-502 (2008-05-27)
Isothiocyanates are phytochemicals with anti-cancer properties that include the ability to trigger apoptosis. A substantial body of evidence suggests that reaction of the electrophilic isothiocyanate moiety with cysteine residues in cellular proteins and glutathione accounts for their biological activity. In
Feng Gao et al.
The Journal of pharmacology and experimental therapeutics, 301(2), 543-550 (2002-04-19)
The present experiment determined the effects of glutathione and ascorbic acid, the two most important hydrophilic antioxidants, on myocardial ischemia-reperfusion injury and evaluated their relative therapeutic values. Isolated rat hearts were subjected to ischemia (30 min) and reperfusion (120 min)
Tünde Pusztahelyi et al.
Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), 30, 96-101 (2014-12-20)
Selenium deficiency is a major health problem worldwide for about 1 billion people. Bacterial cells usually possess low tolerance to selenite stress and also low ability to reduce high concentrations of toxic selenite. Here, high tolerance to selenite and selenium
I P Gontar et al.
Bulletin of experimental biology and medicine, 150(6), 765-769 (2012-01-12)
The proposed method of emulsive polymerization provides the possibility of modifying and obtaining insoluble forms of superoxide dismutase (SOD), glutathione reductase, and streptolysin-O preserving nanoobjects (conformationally active centers and antigenic determinants) in their native states. Apart from enzymatic and immunological
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Questions

1–2 of 2 Questions  

  1. If I have to prepare 1.6 U/ml of glutathione reductase then how should i prepare it (G3664)

    1 answer

    1. The lot-specific Certificate of Analysis provides the units per mg and mg/mL values. Using this information, prepare the glutathione reductase solution in assay buffer immediately before use. This solution should not be stored, and storage of diluted stock solutions is not recommended.

      Helpful?

  2. I recently purchased G3664 (100 units). I am wondering how much volume (mL) of the reagent is there in the bottle. Your response is highly appreciated.

    1 answer

    1. The volume can be determined using the activity value reported on the lot specific Certificate of Analysis.

      For example, if the activity is reported to be 224 units/mg protein (Biuret):
      This equates to 5.2 units /mL protein (Biuret)

      100 units / 224 units/mg =0.446mg, that is, 100 units = 0.446 mg protein
      If the protein content is 5.2 mg/ml, then 0.446mg should be approximately 0.086 ml=86 μL

      Helpful?

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