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包裝
pkg of 1 vial
運輸包裝
ambient
儲存溫度
−20°C
一般說明
The SygRNA synthetic RNA system recruits FnCas9 nuclease to specific double stranded DNA targets. The FnCas9 tracrRNA (trans-activating crRNA) binds to a FnCas9 crRNA (CRISPR RNA) and the resulting crRNA-tracrRNA complex guides FnCas9 protein to crRNA-specified sites in double stranded DNA. SygRNA FnCas9 synthetic tracrRNA and crRNAs are optimized for use with FnCas9 protein and compatible with a variety of nucleic acid delivery and digestion methods. To purchase custom crRNAs click "Request Quotation for Custom crRNA Synthesis" at sigma.com/SygRNA
應用
Functional Genomics/Genome Editing/Molecular Biology/Molecular Cloning/Synthetic Biology
特點和優勢
HPLC-purified SygRNA FnCas9 tracrRNA oligo is optimized for use with target-specific Francisella novicida Cas9 crRNA and FnCas9 protein for genome editing applications, large fragment molecular cloning, and programmable restriction digestion.
外觀
Dry
準備報告
Resuspend SygRNA FnCas9 tracrRNA oligo in RNase-free Tris-containing buffer with a pH 8.0 or less.
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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实验方案
Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.
相关内容
Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.
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