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product name
Minimum Essential Medium Eagle, With Earle′s salts, L-alanyl-glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
品質等級
無菌
sterile-filtered
sterile
形狀
liquid
技術
cell culture | mammalian: suitable
雜質
Endotoxin, tested
成分
stable glutamine: yes
L-glutamine: no
phenol red: yes
sodium pyruvate: no
Earle’s salts (5% CO2): yes
HEPES: no
Hanks’ salts (2% CO2): no
glucose: yes
NaHCO3: yes
運輸包裝
ambient
儲存溫度
2-8°C
一般說明
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.
MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
應用
Minimum Essential Medium Eagle has been used in murine fibroblast culture for in vitro cytocompatibility testing.
其他說明
This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser and L-Gln. It is supplemented with L-Ala-L-Gln dipeptide. This provides a more stable form of glutamine for cell culture. Free amino acid L-glutamine is known to be unstable in cell culture.
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产品编号
说明
价格
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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