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Merck

F6522

Sigma-Aldrich

IgG2a−FITC Isotype Control from murine myeloma

clone UPC-10, purified immunoglobulin, buffered aqueous solution

别名:

IgG2a−Fluorescein isothiocyanate

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

mouse

品質等級

共軛

FITC conjugate

抗體表格

purified immunoglobulin

無性繁殖

UPC-10, monoclonal

形狀

buffered aqueous solution

儲存條件

protect from light

技術

flow cytometry: suitable

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

一般說明

IgG2a (immunoglobulin G 2a) is one of the subclasses of rat immunoglobulins, which also include IgG1, IgG2b and IgG2c.

特異性

Mouse IgG2a, κ, antibodies react with anti-mouse whole serum, anti-mouse IgG2a and anti-mouse κ chain immunoglobulins. The antibody preparation, IgG2a, does not react with antisera to mouse IgA, IgM, IgG1, IgG2b, IgG3 and λ light chain immunoglobulins in Ouchterlony double diffusion assays. IgG2a-FITC conjugate can be used as controls in immunoassays.

應用

IgG2a FITC Isotype Control from murine myeloma has been used in dual-colour staining and in cell staining for flow cytometry.

生化/生理作用

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

準備報告

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Assessment of Contractility of Purified Smooth Muscle Cells Derived from Embryonic Stem Cells
Sinha S, et al.
Stem Cells, 24(7), 1678-1688 (2006)
Three classes and four (sub) classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c
Bazin H, et al.
European Journal of Immunology, 4, 44-48 (1974)
T Nakamura et al.
Proceedings of the National Academy of Sciences of the United States of America, 95(9), 5389-5394 (1998-06-06)
Activation of hepatic stellate (Ito) cells is a final common pathway of liver fibrosis. The findings presented in this paper indicate that expression of Na+/Ca2+ exchanger (NCX) emerges in rat hepatic stellate cells after activation in vitro during primary culture
Expression of the Multiple Sclerosis-Associated MHC Class II Allele HLA-DRB1 * 1501 Is Regulated by Vitamin D
Ramagopalan SV, et al.
PLoS Genetics, 5(2), e1000369-e1000369 (2009)
J Schultz et al.
Cancer research, 60(23), 6663-6669 (2000-12-16)
Single-chain Fv antibody fragments from the CD20-specific murine monoclonal antibody B9E9 were genetically engineered as streptavidin fusions [single-chain Fv-streptavidin (scFvSA) fusion protein] for use in pretargeted radioimmunotherapy. The scFvSA constructs were expressed as soluble, tetrameric species in the periplasm of

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