跳转至内容
Merck
所有图片(1)

文件

F2637

Sigma-Aldrich

Ficoll® 400

BioXtra, for molecular biology, lyophilized powder

别名:

多聚蔗糖 400

登录查看公司和协议定价
所有图片(1)

About This Item

CAS号:
26873-85-8
MDL號碼:
MFCD00081599
分類程式碼代碼:
12352200
NACRES:
NA.52

生物源

non-animal source

等級

Molecular Biology
for molecular biology

產品線

BioXtra

形狀

lyophilized powder

異物活動

DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected

儲存溫度

room temp

正在寻找类似产品? 访问 产品对比指南

相关类别

一般說明

Ficoll® 400是蔗糖的非离子合成聚合物，可用于细胞分离和器官分离。

應用

适用于
  • 杂交实验，以减少标记探针与膜的非特异性结合
  • 制备DNA上样缓冲液（15％ Ficoll®，0.25％溴酚蓝和0.25％二甲苯蓝FF）
  • 分离密度相似但大小不同的细胞（来自人外周血、骨髓和脐带血的PBMC）
  • 产生密度梯度，以便分离亚细胞成分
  • 细胞和组织样品的玻璃化。
  • 比较不同灌注液对缺氧性肺血管收缩（HPV）的影响。
用于细胞分离和细胞器分离。

特點和優勢

  • 不含DNA酶、RNA酶、磷酸酶和蛋白酶
  • 可保留分离细胞的功能和形态完整性

其他說明

一种非离子型合成蔗糖聚合物。

法律資訊

Ficoll is a registered trademark of Cytiva

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Cristina Batlle et al.
Cell reports, 30(4), 1117-1128 (2020-01-30)
Prion-like proteins form multivalent assemblies and phase separate into membraneless organelles. Heterogeneous ribonucleoprotein D-like (hnRNPDL) is a RNA-processing prion-like protein with three alternative splicing (AS) isoforms, which lack none, one, or both of its two disordered domains. It has been
Hae Young Yoo et al.
Pulmonary circulation, 3(2), 396-405 (2013-09-10)
Hypoxic pulmonary vasoconstriction (HPV) is a compensatory physiological mechanism in the lung that optimizes the matching of ventilation to perfusion and thereby maximizes gas exchange. Historically, HPV has been primarily studied in isolated perfused/ventilated lungs; however, the results of these
Gaye Cetinkaya et al.
Cryobiology, 63(3), 292-297 (2011-10-25)
Preservation of cell and tissue samples from endangered species is a part of biodiversity conservation strategy. Therefore, setting up proper cell and tissue cryopreservation methods is very important as these tissue samples and cells could be used to reintroduce the
Ivana Gessara et al.
Stem cell reports, 16(4), 784-796 (2021-03-20)
The ability to genetically manipulate organisms has led to significant insights into functional genomics in many species. In birds, manipulation of the genome is hindered by the inaccessibility of the one-cell embryo. During embryonic development, avian primordial germ cells (PGCs)
Zhaoming Yang et al.
Frontiers in endocrinology, 11, 587450-587450 (2020-12-29)
Islet culture prior to transplantation is a standard practice in many transplantation centers. Nevertheless, the abundant islet mass loss and function impairment during this serum-deprivation culture period restrain the success of islet transplantation. In the present study, we used a
查看所有相关文献

商品

Centrifugation Basics

Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.

Centrifugation Basics

Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.

Centrifugation Basics

Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.

Centrifugation Basics

Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.

实验方案

用于梯度离心的Ficoll® 400

Ficoll 400®是由蔗糖和环氧氯丙烷共聚而成的一种高度分支化的聚合物。Ficoll 400®是完全非离子性的。由于其含有丰富的羟基，Ficoll 400®具有很强的亲水性和水溶性。

Ficoll® 400 for Gradient Centrifugation

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

Ficoll® 400 for Gradient Centrifugation

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

Ficoll® 400 for Gradient Centrifugation

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

查看所有结果

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门