跳转至内容
Merck

F1680

Sigma-Aldrich

Anti-Flotillin 2 (C-terminal)

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

别名:

Anti-ECS, Anti-ESA, Anti-Epidermal surface antigen, Anti-FLOT2, Anti-Reggie-1

登录查看公司和协议定价


About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

rabbit

品質等級

共軛

unconjugated

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

分子量

antigen ~48 kDa

物種活性

human

濃度

~1 mg/mL

技術

indirect immunofluorescence: 5-10 μg/mL using human HEK-293T cells
western blot: 0.5-1 μg/mL using whole extract of human HeLa cells

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... FLOT2(2319)
mouse ... Flot2(14252)
rat ... Flot2(83764)

一般說明

Flotillin-2 is a protein encoded by the FLOT2 gene in humans. Flotillin 2 is more widely distributed, but is absent in skeletal muscle. FLOT2, also called reggie-1, belongs to flotillin protein family integral membrane proteins comprising of prohibitin homology (PHB) domain or SPFH domain (stomatin/prohibitin/flotillin/HflK/C domain).

免疫原

synthetic peptide corresponding to amino acid residues 415-428 of human flotillin 2 with an N-terminal added cysteine, conjugated to KLH. The corresponding sequence differs by 2 amino acids in rat and mouse.

應用

Anti-Flotillin 2 (C-terminal) antibody produced in rabbit has been used in:
  • immunofluorescence
  • immunoprecipitation
  • immunoblotting

生化/生理作用

Flotillin-2 is a protein encoded by the FLOT2 gene in humans. Flotillins are involved in cell adhesion processes and play a role during breast cancer progression. FLOT2 especially plays a functional role in cell-cell adhesion in non-malignant epithelial cells. It may have an essential function in intestinal physiology, especially in the colon. FLOT2 has been implicated in several signaling pathways in tumor cells and can be used as a prognostic biomarker for breast cancer progression.
The prohibitin homology (PHB) domain constitutes a lipid recognition motif which can target proteins to the plasma membrane. The association of flotillin 2 to the plasma membrane depends on palmitoylation and myristolation in a conserved Cys34. Flotillin 2 overexpression is associated with melanoma progression and transformation of SB2 melanoma cells to a highly metastatic line. It is also associated with expression of the thrombin receptor, protease activated receptor 1 (PAR-1), that is implicated in cell growth and metastasis. Flotillin 2 may be used as a marker protein for lipid raft microdomains.

標靶描述

Flotillin 2 (C-terminal) encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling.

外觀

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

未找到合适的产品?  

试试我们的产品选型工具.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Benedikt Beckert et al.
International journal of molecular sciences, 20(13) (2019-06-30)
Pemphigus Vulgaris is an autoimmune disease that results in blister formation in the epidermis and in mucosal tissues due to antibodies recognizing desmosomal cadherins, mainly desmoglein-3 and -1. Studies on the molecular mechanisms of Pemphigus have mainly been carried out
Membrane and raft association of reggie-1/flotillin-2: role of myristoylation, palmitoylation and oligomerization and induction of filopodia by overexpression
Neumann-Giesen C, et al.
The Biochemical Journal, 378(2), 509-518 (2004)
Annika Gauss et al.
International journal of medical sciences, 10(10), 1259-1270 (2013-08-29)
The etiopathogenesis of inflammatory bowel diseases (IBD) remains largely unexplained. Flotillins (flotillin-1 and flotillin-2) are ubiquitous proteins which have been linked to inflammation and regeneration. We hypothesized that alterations in the expression of flotillin-2 in enterocytes may be related to
Immortalized Human hTert/KER-CT Keratinocytes a Model System for Research on Desmosomal Adhesion and Pathogenesis of Pemphigus Vulgaris
Beckert B, et al.
International Journal of Molecular Sciences, 20(13), 3113-3113 (2019)
Differences in the expression and distribution of flotillin-2 in chick, mice and human muscle cells
Possidonio ACB, et al.
PLoS ONE, 9(8), e103990-e103990 (2014)

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门