This product is not tested for in vivo use. The Quality testing does include neurite formation in cell culture. The end user would have to determine suitability for use in animal models. This ECM product undergoes an additional purification step which depleted growth factors. However, the material is not tested for the presence of growth factors post-purification. See the links below to review additional information regarding the presence and depletion of growth factors in Extracellular Matrix from mouse sarcoma:
https://www.researchgate.net/publication/41454358_Matrigel_A_complex_protein_mixture_required_for_optimal_growth_of_cell_culture
https://www.pnas.org/doi/epdf/10.1073/pnas.87.10.4002
https://www.sciencedirect.com/science/article/pii/S1044579X05000313#bib16
https://www.sciencedirect.com/science/article/abs/pii/001448279290397Q
推荐产品
质量水平
产品线
BioReagent
表单
liquid
浓度
7-9 mg/mL
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−20°C
基因信息
mouse ... Hspg2(15530) , Lama1(16772) , Lama3(16774) , Lama5(16776) , Lamb1-1(16777) , Lamc1(226519) , Lamc2(16782) , Nid1(18073) , Sdc2(15529)
正在寻找类似产品? 访问 产品对比指南
应用
生化/生理作用
用于生产本品的每个小鼠种群都定期筛查病原体。经检测，LDEV呈阴性。
组分
生长因子减少的修改可为培养基提供更多的控制，更好地解释结果并提高聚合度。 该培养基还缺少可能会干扰某些分析的酚红。
注意
制备说明
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
商品
Cell culture protocols using ECM Gel Matrix EHS basement membrane extract (BME) for neurite outgrowth, cell invasion and angiogenesis tube formation assays.
Cell culture protocols using ECM Gel Matrix EHS basement membrane extract (BME) for neurite outgrowth, cell invasion and angiogenesis tube formation assays.
Cell culture protocols using ECM Gel Matrix EHS basement membrane extract (BME) for neurite outgrowth, cell invasion and angiogenesis tube formation assays.
Cell culture protocols using ECM Gel Matrix EHS basement membrane extract (BME) for neurite outgrowth, cell invasion and angiogenesis tube formation assays.
-
ECM cat:E6909 I would like to know if this product could be used in vivo to inject tumor cells into mouse liver.Its description says reduced growth factor but I would like to know if it contains anything, even in a low %,that can induce cell proliferation
1 answer-
Helpful?
-
-
What is the application of ECM for tube assay and the impact of reduced growth factors?
1 answer-
I recommend the following publication for a comparison study on ECM vs ECM with reduced growth factor: J Vis Exp. 2016 Aug 14;(114):54074. doi: 10.3791/54074. Strategic Endothelial Cell Tube Formation Assay: Comparing Extracellular Matrix and Growth Factor Reduced Extracellular Matrix. PMID: 27585062.
J Vis Exp. 2016 Aug 14;(114):54074. doi: 10.3791/54074. Strategic Endothelial Cell Tube Formation Assay: Comparing Extracellular Matrix and Growth Factor Reduced Extracellular Matrix. PMID: 27585062.
Helpful?
-
-
Is it possible to dissociate cells using Accutase when they are attached to the ECM gel E6909?
1 answer-
Yes, it is possible to dissociate cells using Accutase when they are attached to the ECM gel E6909. It is advisable to start with a small-scale trial to assess its suitability.
Helpful?
-
-
Do Products E1270 and E6909, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, contain phenol red?
1 answer-
Product E1270 ECM gel does contain phenol-red from the Dulbecco's modified Eagle's medium component of the product. Product No. E6909, ECM gel, growth factor reduced, is phenol red free.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
Are ECM Gels, Products E1270 and E6909, the same as Matrigel (from BD)?
1 answer-
Product No. E1270, ECM gel is the equivalent of Matrigel from BD only produced by Sigma-Aldrich.
Helpful?
-
-
Are Products E1270 and E6909, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, growth factor reduced like Matrigel (from BD)?
1 answer-
Product No. E1270 is not growth factor reduced. Product No. E6909 is ECM gel, growth factor reduced.
Helpful?
-
-
How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
Helpful?
-
Active Filters
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门
