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E4274

内毒素去除溶液

Name: 内毒素去除 溶液
Brand: SIGMA

Remove lipopolysaccharides (LPS) from plasmid DNA preparations

别名:

ET removal buffer, ET removal reagent, ET removal solution, endo removal buffer, endo removal reagent, endo removal solution, endotoxin contamination removal, endotoxin removal buffer, endotoxin removal reagent, plasmid purification endo, plasmid purification endo free

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About This Item

UNSPSC代码:

12352200

NACRES:

NA.52

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质量水平

200

应用

内毒素去除溶液已被用来去除面包酵母核糖核酸中的脂多糖。

生化/生理作用

该试剂依赖于Triton X-114的胶束性质来去除溶液中的内毒素。当温度高于20°C时，胶束聚集成一个单独的相，隔离两亲性溶质，包括脂多糖。亲水DNA保留在水相中。

数量

1体积处理5体积的脱氧核糖核酸溶液（& # 8804；1毫克/毫升）

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Determination of endotoxin through an aptamer-based impedance biosensor.

Su W

Biosensors And Bioelectronics, 32(1), 32-36 (2012)

Lipopolysaccharide is a frequent contaminant of plasmid DNA preparations and can be toxic to primary human cells in the presence of adenovirus.

M Cotten et al.

Gene therapy, 1(4), 239-246 (1994-07-01)

Endotoxin (lipopolysaccharide, LPS) is commonly found as a contaminant in plasmid DNA preparations. We demonstrate here that the quantities of LPS typically contaminating DNA preparations can generate a toxicity to primary cells (primary human skin fibroblasts, primary human melanoma cells)

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Cell Culture FAQs: Bacterial Endotoxin Contamination

FAQs on bacterial endotoxin contamination, details on endotoxin testing using the LAL assay, and tips to avoid contamination in cell cultures.

Cell Culture FAQs: Bacterial Endotoxin Contamination

FAQs on bacterial endotoxin contamination, details on endotoxin testing using the LAL assay, and tips to avoid contamination in cell cultures.

Cell Culture FAQs: Bacterial Endotoxin Contamination

FAQs on bacterial endotoxin contamination, details on endotoxin testing using the LAL assay, and tips to avoid contamination in cell cultures.

Cell Culture FAQs: Bacterial Endotoxin Contamination

FAQs on bacterial endotoxin contamination, details on endotoxin testing using the LAL assay, and tips to avoid contamination in cell cultures.

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内毒素去除溶液