DUO96030

Duolink^® PLA Multicolor Probemaker Kit - Orange

Proximity Ligation Assay Multiplexing: protein-protein interaction, post-translational modification and low abundant protein events

• 别名: in situ Proximity Ligation Assay Kit, Protein Protein Interaction Kit
• 分類程式碼代碼: 41105331
• NACRES: NA.32

属性

• 產品線: Duolink^®
• 技術: immunofluorescence: suitable; multiplexing: suitable; proximity ligation assay: suitable
• 螢光: λ_ex 542 nm; λ_em 562 nm (orange); λ_ex 542 nm; λ_em 562 nm
• 適合性: suitable for fluorescence
• 運輸包裝: dry ice
• 儲存溫度: −20°C

说明

應用

Specificity
Orange fluorescence detection reagents are often used with Cy^®3 filter.

Application Note
Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink^® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink^® projects

View full Duolink^® product list

特點和優勢

Multicolor Duolink^® proximity ligation assay (PLA^®) is an application of the Duolink^® technology that allows for endogenous, simultaneous detection of up to 4 protein interactions, post-translational modifications, or protein expression levels (including low abundant protein) in fixed cells and tissue samples. Follow the Duolink^® PLA Probemaker Guide for Multicolor Detection and the Duolink^® PLA Multicolor Detection protocol to use this product.

• Visualize and detect multiple proteins events in one experiment.
• No overexpression or genetic manipulation required
• High specificity (fewer false positives)
• Detection of low abundant proteins due to rolling circle amplification
• Relative quantification possible
• No special equipment needed
• Faster and more simple than FRET
• Increased accuracy compared to co-IP
• Publication-ready results

Components
Duolink^® PLA Multicolor Probemaker Kit - Orange contains all the necessary reagents to perform the conjugations of Orange Oligo F and Orange Oligo G directly to primary antibodies. The resulting PLA probes (Orange F and Orange G) must be used together and cannot be paired with any other Duolink^® PLA probe.

This product is comprised of the following:

• Orange Oligo F DUO86030F
• Orange Oligo G DUO86030G
• Conjugation Buffer DUO82033
• Stop Reagent DUO82034
• Storage Solution DUO82035

See datasheet for more information.

To perform a Multicolor Duolink^® PLA experiment primary antibodies (PLA, IHC, ICC or IF validated) that recognize protein targets of interest are required. Duolink^® PLA Multicolor Probemaker-generated probes (Red A/Red B, Green C/Green D, Orange F/Orange G, and/or FarRed H/FarRed I) are used in combination to perform the Multiplex experiment.The reagents in the Duolink^® PLA Multicolor Reagent Pack are required to perform the amplification and detection of the resulting PLA probes. Required additional reagents include Duolink^® Wash Buffers and Mounting Medium.

法律資訊

Cy is a registered trademark of Cytiva

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany

PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 象形圖: GHS07,GHS09
• 訊號詞: Warning
• 危險聲明: H317,H411
• 防範說明: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• 危險分類: Aquatic Chronic 2 - Skin Sens. 1
• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3