OV56

96020759, human ovary, Epithelial

• 别名: OV-56 Cells
• UNSPSC代码: 41106514

属性

• 产品名称: OV56, 96020759
• 生物来源: human ovary
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Epithelial
• 产品: VEGF
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human ovarian serous carcinoma (ascites) stage IV, poorly differentiated

细胞系描述

The OV56 cell line was established from ascitic fluid derived from a 46-year-old female patient diagnosed with ovarian cancer. The tumour was characterised as stage IV, poorly differentiated. Immunocytochemical staining of the cells was reported to show strong staining for cytokeratin (CK), weak staining for carcinoembryonic antigen (CEA) and strong staining for human milk fat globulin (HMFG-2). Production of vascular endothelial growth factor (VEGF) has been detected by ELISA. The patient had received sequential treatment with cisplatin and cyclophosphamide, cisplatin and etoposide and subsequently tamoxifen.

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 8,9
D16S539: 10,13
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 9,11
vWA: 15,19

培养基

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) + 0.5 ug/ml hydrocortisone + 10ug/ml insulin

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他说明

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