M15

95102517, mouse kidney, Epithelial

• UNSPSC代码: 41106514

属性

• 产品名称: M15, 95102517
• 生物来源: mouse kidney
• 生长模式: Adherent
• 核型: Hyperdiploid
• 形态学: Epithelial
• 产品: WT1 (expressed gene)
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Mouse mesonephric epithelium, polyoma virus large T transformed

细胞系描述

The WT1-expressing mesonephric cell line M15 (alias Meso15) was established from mouse mesonephros transgenically expressing the large T protein of polyoma virus under the control of the early viral enhancer. As a tumour suppresser gene with a key role in urogenital development, WT1 is implicated as predisposition gene in the pathogenesis of Wilms′ tumour (WT).

应用

Gene expression and protein studies connected to kidney development and Wilms′ tumourigenesis.

培养基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. NB: Cell growth is impaired at lower densities.

其他说明

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