GP2d

95090714, human colon, Epithelial

• 别名: GP2d Cells
• 分類程式碼代碼: 41106514

属性

• product name: GP2d, 95090714
• 生物源: human colon
• 增長模式: Adherent
• 染色體組型: Diploid
• 形態學: Epithelial
• 產品: Not specified
• 受體: Epidermal growth factor (EGF)
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian colon adenocarcinoma

細胞系描述

GP2d has been established from the same adenocarcinoma as GP5d (Sigma Catalogue number. 95090715). This has been confirmed by STR profiling at ECACC. The cells were derived from a local recurrence of Duke′s grade B, poorly differentiated carcinoma of the colon from a 71 year old female at surgical resection. The patient received no preoperative chemo- or radiotherapy and had a strong family history of colon cancer with two first degree relatives dying of the disease below the age of 55 years. Both clones possess genetic changes consistent with the pattern of tumour progression in colon cancer but are morphologically distinct. An interstitial deletion on chromosome 5 (region 14q to 22q) and an inverted duplication of bands 10q11 and 10q21 has been reported. Both cell lines contain a normal copy of the ki-ras gene and a copy with a transition in codon 12. GP2d cells show growth response to EGF, TGF α or insulin with an increase in cell numbers. Amphiregulin mRNA is abundant in GP2d but almost undetectable in GP5d.

應用

GP2d has been used to extract genomic DNA for digital PCR (dPCR). It has also been used to extract DNA from the cell with PIK3CA mutation c.3140A>T.

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 9,12
D13S317: 8,12
D16S539: 11,13
D5S818: 11,12
D7S820: 9,11
THO1: 7,9.3
TPOX: 8,11
vWA: 17,18

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 5x10,000 to 1x100,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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