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product name
SK-N-AS, 94092302
生物源
human nerve (bone marrow metastasis)
增長模式
Adherent
染色體組型
46
形態學
Polygonal
產品
Not specified
受體
Vimentin
技術
cell culture | mammalian: suitable
相關疾病
metastasis
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Human neuroblastoma
細胞系描述
Derived from a female patient with neuroblastoma from the metastasis of the bone marrow. Tumourigenic in nude mice.
應用
Neuroblastoma differentiation studies
DNA分析
STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 9
D16S539: 14
D5S818: 11,12
D7S820: 11,13
THO1: 9.3
TPOX: 11,12
vWA: 16,17
CSF1PO: 10,12
D13S317: 9
D16S539: 14
D5S818: 11,12
D7S820: 11,13
THO1: 9.3
TPOX: 11,12
vWA: 16,17
培養基
DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
其他說明
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