PSN1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC代码: 41106514

属性

• 生物来源: human pancreas
• 包装: tube of 5 μg 94060601-DNA-5UG; pkg of vial of cells 94060601-1VL
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Epithelial
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human pancreatic adenocarcinoma

细胞系描述

The human pancreatic cancer cell line PSN1 was derived from pancreatic adenocarcinoma tissue. After transplantation into nude mice the PSN1 cell line was established from the xenograft. PSN1 cells are unique in that amplification of both c-myc (50-fold) and activated c-Ki-ras (3-6 fold) with a point mutation from GGT to CGT at codon 12 are present in the same degree as in the original tumour and contain increased amounts of c-myc and c-Ki-ras transcripts. Loss of one of the two p53 alleles and a point mutation from AAG to CAG at codon 132 in the remaining allele was also observed in PSN1.

应用

PSN1 has been used to study synthetic acetylhomoagmatine in vitro. It has also been used to determine the cytotoxicity of irinotecan and topotecan.

PSN1 is an example of a cancer cell having multiple genetic changes including those of two oncogenes (c-Ki-ras and c-myc) and a tumour suppresser gene (p53).

培养基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他说明

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