TGP 54

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC代码: 41106514

属性

• 生物来源: mouse pancreas
• 包装: tube of 5 μg 94022430-DNA-5UG; pkg of vial of cells 94022430-1VL
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Not specified
• 产品: Somatostatin
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Mouse pancreatic islet cell tumour

细胞系描述

TGP 54 has been isolated from an islet tumour arising in the pancreas of a transgenic Tg (E1a-1-SV40E)Bri8 mouse. These mice carry a pseudogene construct composed of a pancreatic-specific elastase-1 promoter linked to the SV40T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 54 cells are SV40T antigen positive and secrete low amounts of somatostatin, it is one of a series of cells obtained from pancreatic tumours deposited at ECACC.

应用

Study of exocrine pancreas carcinomas, hormone secretion studies.

培养基

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他说明

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