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product name
QT 6, 93120831
生物源
quail muscle
增長模式
Adherent
染色體組型
Not specified
形態學
Spindle shaped/round
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Quail Japanese fibrosarcoma
細胞系描述
Derived from methylcholanthrene-induced fibrosarcoma of Japanese quail. The cells are susceptible to several viruses including sub group A avian sarcoma, avian encephalomyelitis, Marek′s disease herpes virus and rabies virus.
應用
Virus studies
培養基
Ham′s F10 + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C; subculture at about 75% confluency; cells can be dislodged by tapping flask against the palm of hand; use of 0.5% polyvinylpyrrol
其他說明
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