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产品名称
G-402, 90112715
生物来源
human kidney
生长模式
Adherent
核型
Modal no. 47
形态学
Epithelial-like
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
细胞系来源
Human Caucasian renal leiomyoblastoma
细胞系描述
Derived from a tumour of a 9 month old female Caucasian. Highly transformed and grows in soft agar.
应用
Transformation and tumourigenicity studies
DNA图谱分析
STR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 9,12
D16S539: 11,13
D5S818: 11,12
D7S820: 9,10
THO1: 7,9
TPOX: 10
vWA: 13,17
CSF1PO: 10,13
D13S317: 9,12
D16S539: 11,13
D5S818: 11,12
D7S820: 9,10
THO1: 7,9
TPOX: 10
vWA: 13,17
培养基
McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Change medium every 4 days.
其他说明
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