MLg

90112707, mouse lung, Fibroblast-like

• 分類程式碼代碼: 41106514

属性

• product name: MLg, 90112707
• 生物源: mouse lung
• 增長模式: Adherent
• 染色體組型: Modal no. 64; hypodiploid
• 形態學: Fibroblast-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse ddY lung

細胞系描述

Established from normal lung tissue of a new-born, ddY strain mouse. Both induced and spontaneous release of C-type particles have been reported.

應用

Virus studies

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) or New Born Calf Serum (NBCS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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