RTG-2

90102529, fish ovary, Fibroblast

• 分類程式碼代碼: 41106514

属性

• product name: RTG-2, 90102529
• 生物源: fish ovary
• 增長模式: Adherent
• 染色體組型: 2n = 60
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Trout Rainbow gonad tissue

細胞系描述

From pooled male/female gonad tissue of yearling rainbow trout (Oncorhynchus mykiss). First cell line to be established from cold-blooded vertebrates. 8-fold difference in growth rate in temperature range 4-24°C. Susceptible to infectious pancreatic necrosis virus, Egtved virus and arboviruses (EEE, VEE).

應用

Virus studies: Inf. pacr. necrotic virus, Egtved virus, arboviruses (EEE, VEE)

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Passage using 0.25% trypsin below 24°C. Cells may be seeded as few as 3x10,000 cells/ml 5% CO₂; 24°C. Fish cell lines detach easily during transit if the culture is very youn

其他說明

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