BF-2

87032603, fish trunk, Fibroblast

• 分類程式碼代碼: 41106514

属性

• product name: BF-2, 87032603
• 生物源: fish trunk
• 增長模式: Adherent
• 染色體組型: 2n = 48
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Fish Bluegill fry caudal trunk

細胞系描述

Derived from a trypsinised suspension of pooled caudal portions of the trunk of one year old fingerlings. Will grow at temperatures from 15°C to 33°C. Cells susceptible to lymphocystis virus of fish and tadpole edema virus (TEV) of frogs.

應用

Virus studies, targets for bacterial cytotoxins

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (pH7.4 - 7.5).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin/EDTA; 5% CO₂; 23°C. A replication capacity of up to 50 passages is expected. Fish cell lines detach easily during transit if the culture is too young. After resuscitation, split cells

其他說明

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