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product name
SCP, 89101302
生物源
ovine brain
增長模式
Adherent
染色體組型
Not specified
形態學
Not specified
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
運輸包裝
dry ice
儲存溫度
−196°C
相关类别
細胞系來源
Ovine brain choroid plexus
細胞系描述
Initiated from the choroid plexus of an ovine brain. The cells support propagation of Visna virus, strain 1514 with resulting cytopathic effects. Tests have indicated that the cell line produces both detectable BVDV antigens and infectious BVDV.
應用
Virus studies: Visna virus
培養基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他說明
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