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Jurkat E6.1 Cell Line human

88042803, human blood (leukemic T-cell lymphoblast), Not specified

别名:

Jurkat Cells, Jurkat Clone E6-1 Cells

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About This Item

UNSPSC代码:
41106514

产品名称

Jurkat E6.1 Cell Line human, 88042803, from human blood(leukemic T-cell lymphoblast)

生物来源

human blood (leukemic T-cell lymphoblast)

生长模式

Suspension

核型

Pseudodiploid, modal No 46

形态学

Not specified

产品

Interleukin 2 (IL-2), human alpha interferon

受体

Not specified

技术

cell culture | mammalian: suitable

相关疾病

cancer

运输

dry ice

储存温度

−196°C

相关类别

细胞系来源

Human leukaemic T cell lymphoblast

细胞系描述

Derived from Jurkat FHCRC. An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages.

应用

IL-2 assay and cloning studies
Jurkat E6.1 cell line has been used to:
  • study cell-independent/vector-free delivery of bovine serum albumin-fluorescein conjugate
  • determine the cytotoxicity of the cytolethal distending toxin (CDT) holotoxin and its components
  • study the expression of bisphenol A exposed estrogen receptor-β (ERβ) and estrogen-related-receptor-α (ERRα) in vitro

DNA图谱分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18

培养基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. On resuscitation single cells can be observed, during culture most cells will grow as aggregates.

其他说明

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历史批次信息供参考:

分析证书(COA)

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实验方案

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Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.

T-Cell Migration Assays Using Millicell® Cell Culture Inserts

Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.

T-Cell Migration Assays Using Millicell® Cell Culture Inserts

Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.

T-Cell Migration Assays Using Millicell® Cell Culture Inserts

Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.

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