FM3Ats C1.T85

87111904, mouse breast, Lymphoblast

• UNSPSC代码: 41106514

属性

• 产品名称: FM3Ats C1.T85, 87111904
• 生物来源: mouse breast
• 生长模式: Suspension
• 核型: Not specified
• 形态学: Lymphoblast
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Mouse C3H mammary carcinoma

细胞系描述

A temperature sensitive mutant isolated from the mouse mammary cell carcinoma cell line, FM3A (Sigma Catalogue number. 87100804). Cells will not divide at 39°C (non-permissive temperature) and will grow at 33°C (permissive temperature). Electron microscope findings have shown that chromatin abnormally condenses into fragmented and compact forms in 80% of the cells at 39°C for 16 hours. This abnormal condensation does not occur at 33°C or in the FM3A cell line at either temperature.

应用

Tumourigenicity studies, chromosome condensation studies.

培养基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Maintain cultures between 1-3x100,000 cells/ml; 5% CO₂; 33°C.

其他说明

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