MPK

87032604, mini pig kidney, Fibroblast

• 分類程式碼代碼: 41106514

属性

• product name: MPK, 87032604
• 生物源: mini pig kidney
• 增長模式: Adherent
• 染色體組型: 2n = 38, diploid
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Minipig kidney

細胞系描述

Established from the kidney of a 118 day old foetal minipig. The cells are susceptible to some arboviruses and leptospiras and highly refractory to Chlamydia spp. and meningopneumonitis.

應用

Virus studies: arboviruses

培養基

EMEM (EBSS) + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 20% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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