Detroit 551

86082802, human skin (embryonic), Fibroblast

• UNSPSC代码: 41106514

属性

• 产品名称: Detroit 551, 86082802
• 生物来源: human skin (embryonic)
• 包装: tube of 5 μg 86082802-DNA-5UG; pkg of vial of cells 86082802-1VL
• 生长模式: Adherent
• 核型: 2n = 46
• 形态学: Fibroblast
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human Caucasian embryo skin

细胞系描述

Derived from a skin biopsy of a normal Caucasian female embryo. Cells may be considered normal and may serve as controls for study of cells having mutant chromosomes. Cells support growth of herpes viruses and SV40 virus can induce transformation.

应用

Chromosome and virus studies (Normal control): herpesviruses, SV40

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 12,13
D5S818: 12,13
D7S820: 10,12
THO1: 9,9.3
TPOX: 8
vWA: 18

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan; at most 25 subcultures from original tissue.

其他说明

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