B82

85011408, mouse adipose tissue, Fibroblast

• 分類程式碼代碼: 41106514

属性

• product name: B82, 85011408
• 生物源: mouse adipose tissue
• 增長模式: Adherent
• 染色體組型: 2n = 51
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse C3H/An sc areolar and adipose tissue

細胞系描述

Derivative of NCTC clone 929 (Sigma Catalogue number. 88102702) obtained by progressive selection in BUdR, has a cloning efficiency of 70-80%.

應用

Chromosome analysis and hybridisation studies

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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