MDCK Cell Line canine

84121903, canine kidney, Epithelial

• 别名: MDCK (NBL-2) Cells, Madin Darby Canine Kidney Cells, NBL-2 Cells
• 分類程式碼代碼: 41106514
• eCl@ss: 32190102

属性

• product name: MDCK Cell Line canine, 84121903
• 生物源: canine kidney
• 形狀: cell suspension
• 增長模式: Adherent
• 染色體組型: 2n = 78
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Canine Cocker Spaniel kidney

細胞系描述

From kidney of normal female adult Cocker Spaniel in 1958 by SH Madin and NB Darby (Madin Darby Canine Kidney). Supports growth of wide range of animal viruses: VSV (Indiana strain), infectious Canine Hepatitis, Vaccinia, Coxsackie B5, Adeno and reo viruses, SVEV.

應用

Virus studies: SVEV, VSV, Vaccinia, adeno and reoviruses, Coxsackie B5 et al

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.

其他說明

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