LA-N-1

6041201, human nerve, Tear-drop shaped

• 别名: LAN 1 Cells, LAN1 Cells
• 分類程式碼代碼: 41106514

属性

• product name: LA-N-1, 06041201
• 生物源: human nerve
• 增長模式: Semi-adherent aggregates
• 染色體組型: Modal no. 87, range 47 - 87
• 形態學: Tear-drop shaped
• 產品: Catecholamine
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: metastasis
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Neuroblastoma Bone Marrow Metastasis

細胞系描述

Established by Seeger et al., (1977) from neuroblastoma cells in the bone marrow of a 2-year-old male with clinical Stage IV neuroblastoma. The cells are neuroblastic, tear-drop shaped with multiple short fine cell processes (neurites). The cells are tumourigenic in nude mice. This is a catecholamine producing neuroblastoma cell line.LA-N-1 (Sigma Catalogue number 06041201), LA1-55n (Sigma Catalogue number 06041203) and LA1-5s (Sigma Catalogue number 06041204) have been shown to originate from the same patient by STR profiling.

Established by Seeger et al., (1977) from neuroblastoma cells in the bone marrow of a 2-year-old male with clinical Stage IV neuroblastoma. The cells are neuroblastic, tear-drop shaped with multiple short fine cell processes (neurites). The cells are tumourigenic in nude mice. This is a catecholamine producing neuroblastoma cell line.LA-N-1 (Sigma Catalogue number 06041201), LA1-55n (Sigma Catalogue number 06041203) and LA1-5s (Sigma Catalogue number 06041204) have been shown to originate from the same patient by STR profiling.

DNA分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11,12
D16S539: 9
D5S818: 12
D7S820: 10,11
THO1: 8,9.3
TPOX: 8,11
vWA: 16,19

培養基

EMEM (with non-essential amino acids) and Ham′s F12 (1:1 mixture) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

例行更新培養

For routine maintenance, split cultures only after they have become very dense i.e. split once every 2-3 weeks at a 1:20 - 1:100 ratio; 8% CO₂; 37°C. Cells grow best and are most adherent on a plastic substrate in medium at a pH of 6.9 - 7.2; they do not tolerate more alkaline pH well. Population doubling time is approximately 2 days; saturation density is >1,000,000 cells/cm². Cells grow in weakly adherent clusters. Allow all floating clumps to settle and withdraw most of the medium. Remove attached cells from substrate with 0.1% trypsin or PBS alone, cells will detach in <5 minutes. Remaining cells, if any, are large, flat and tightly adherent precursors of Schwann cell/glia/melanocyte lineages. If desired they should be removed from the substrate with trypsin/EDTA. When the neuronal cells are seeded into a new flask the cells attach slowly and may remain in suspension for one to several days; do not change the medium the day after passage. Floating clumps of cells are viable. This passage schedule will select for cells retaining a neuroblastic phenotype; cultures transferred more frequently, with larger inocula, and/or with trypsin/EDTA may gradually lose their neuroblastic properties due to overgrowth by spontaneously arising, non-neuronal adherent cell variants. When cells are resuscitated from a frozen ampoule the cells may appear dead after a day, but reattach and resume growth within 2-3 days reaching confluence by 7 days.

其他說明

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