NS0-Turbodoma

6020202, mouse blood, lymphoblast

• 分類程式碼代碼: 41106514

属性

• product name: NS0-Turbodoma, 06020202
• 生物源: mouse blood
• 增長模式: Not specified
• 染色體組型: Not specified
• 形態學: lymphoblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse myeloma, serum-free, without cholesterol

細胞系描述

NS0 cell line (Sigma Catalogue no. 85110503) adapted to grow in the chemically defined protein- and peptide-free medium Turbodoma (Cell Culture Technologies). Cholesterol is not required as a supplement for this culture. This cell line is suitable for use as a fusion parent for serum-free generation of hybridomas. The NS0 cell line is the property of the Medical Research Council (MRC). ECACC is unable to supply this cell line or derivatives of this cell line to commercial entities unless a license has been first secured from LifeArc (formerly known as MRC Technology). Requests for commercial use of this cell line will be forwarded to LifeArc.

培養基

Turbodoma medium (Cell Culture Technologies) + 2mM L-Glutamine (G7513). When freezing cells down use TurboDoma^™ medium (50:50 conditioned medium:fresh medium) + 2mM L-Glutamine (G7513) + 10% DMSO.

例行更新培養

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法律資訊

TurboDoma is a trademark of Cell Culture Technologies

免責聲明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.