Sf21

5022801, insect ovary, Spherical

• UNSPSC代码: 41106514

属性

• 产品名称: Sf21, 05022801
• 生物来源: insect ovary
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Spherical
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Spodoptera frugiperda pupal ovarian tissue

细胞系描述

Derived from pupal ovarian tissue of spodoptera frugiperda. The cells are highly susceptible to Baculovirus infection and are used in the production of protein products genetically manipulated into Baculovirus vector systems. They were derived at the USDA Insect Pathology Laboratory from a cell line originating from pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. Sf21 cells have greater range in size and have a doubling time of 24 hr compared to 48-72 hr for Sf9 cells.

应用

Protein expression using the Baculovirus vector system

培养基

SF90011 + 10% Foetal Bovine Serum (FBS)

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 5x10,000 cells/cm²; 28 C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, centrifuge suspension and resuspend cell pellet in fresh medium.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.