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Merck
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C9830

Sigma-Aldrich

壳聚糖酶 来源于灰色链霉菌

lyophilized powder, >50 units/mg protein (Bradford)

别名:

壳聚糖N-乙酰氨基葡糖水解酶

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About This Item

CAS号:
51570-20-8
EC 号:
3.2.1.132 (BRENDA, IUBMB)
MDL號碼:
MFCD00211797
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

lyophilized powder

品質等級

200

比活性

>50 units/mg protein (Bradford)

純化經由

chromatography

溶解度

H2O: soluble, clear to slightly hazy, colorless (10U/0.3mL)

異物活動

chitinase <1.0 units/mg protein

儲存溫度

−20°C

相关类别

一般說明

壳聚糖酶作为细胞外分泌酶由灰链霉菌 合成，其分子量为34 kDa。它的最适温度和pH分别为60°C和5.7。壳聚糖酶属于糖基水解酶家族，并包含α-螺旋三级结构，类似于溶菌酶，在两瓣之间存在与底物结合的裂隙。

應用

Sigma的酶已被用于水解卵菌根腐丝囊霉Aphanomyces euteiches（豆科植物的主要寄生虫）的细胞壁粗制品。它还被用于完全脱-N-乙酰化壳聚糖的酶促水解，以便在制备生物相容性壳聚糖-藻酸盐凝胶时得到壳聚糖低聚物混合物。
来自灰链霉菌的壳聚糖酶已被用于消化泪腺（LG）组织。
来自灰色链霉菌（Streptomyces griseus）的壳聚糖酶已被用于评估几丁质来源对几丁质酶和壳聚糖酶产生的影响。来自灰色链霉菌（Streptomyces griseus）的壳聚糖酶还被用于研究：通过使用胶体几丁质和不同程度的壳聚糖脱乙酰化来研究灰色链霉菌（Streptomyces griseus）HUT 6037的几丁质酶和壳聚糖酶的有效产生。

生化／生理作用

壳聚糖酶可在N-乙酰-D-氨基葡萄糖和D-氨基葡萄糖残基之间的β（1,4）键处催化N-乙酰化壳聚糖的内水解。来自灰链霉菌的壳聚糖酶能够水解壳聚糖和羧甲基纤维素。壳聚糖酶存在于多种类型的微生物中，并可用于真菌细胞壁的裂解。

單位定義

根据Osswald等(1992)描述的荧光测定法，一个酶活性单位是指在pH 5.0、37℃下，每分钟催化壳聚糖释放1 μmole葡萄糖胺所需的酶量。

外觀

含磷酸钾缓冲盐的冻干粉

準備報告

对于长期溶液储存，应使用50％甘油溶液重悬样品瓶内容物。用0.1％BSA溶液稀释至工作浓度。

其他說明

更多关于复杂碳水化合物分析酶的信息请见www.sigma-aldrich.com/enzymeexplorer

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H334

防範說明

P261 - P284 - P501

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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Toshiaki Tanabe et al.
Bioscience, biotechnology, and biochemistry, 67(2), 354-364 (2003-05-06)
Streptomyces griseus HUT 6037 inducibly produced two chitosanases when grown on chitosan. To elucidate the mechanism of degradation of chitinous compound by this strain, chitosanases I and II of S. griseus HUT 6037 were purified and characterized. The purified enzymes
Pascal Viens et al.
Marine drugs, 13(11), 6566-6587 (2015-11-01)
Chitosanases, enzymes that catalyze the endo-hydrolysis of glycolytic links in chitosan, are the subject of numerous studies as biotechnological tools to generate low molecular weight chitosan (LMWC) or chitosan oligosaccharides (CHOS) from native, high molecular weight chitosan. Glycoside hydrolases belonging
Georg Kopplin et al.
RSC advances, 11(23), 13780-13798 (2022-04-16)
Three alginates with fundamentally different block structures, poly-M, poly-G, and poly-MG, have been investigated upon ionic crosslinking with chitosan oligosaccharides (CHOS), using circular dichroism (CD), rheology, and computer simulations, supporting the previously proposed gelling principle of poly-M forming zipper-like junction
Thang Trung Khong et al.
Biomacromolecules, 14(8), 2765-2771 (2013-06-29)
Biocompatible hydrogels are very interesting for applications in, e.g., tissue engineering and for immobilization of cells, such as calcium-alginate gels where the calcium ions form specific interactions with the guluronic acid units. We here report on a new gelling system
Chitosanase from Streptomyces griseus.
A Ohtakara
Methods in enzymology, 161, 505-510 (1988-01-01)
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