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Key Documents

C-12917

Sigma-Aldrich

Human M2 Macrophages (hMDM-MCSF(-))

Monocyte-derived, single donor, 5 million cryopreserved cells

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About This Item

分類程式碼代碼:
41106514
NACRES:
NA.81

生物源

human blood (monocyte)

包裝

pkg of 500,000 cells

形態學

(macrophages)

技術

cell culture | mammalian: suitable

運輸包裝

dry ice

儲存溫度

−196°C

一般說明

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

細胞系來源

Monocyte

應用

Cryopreserved human monocyte-derived macrophages (hMDM) are convenient and easy-to-handle. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks. Optionally, user-customizable activation of the cells can be performed.Cryopreserved human macrophages are produced from monocytes in M2 Macrophage Generation Media DXF and are available as non-activated, fully qualified M2- (M-CSF) polarized cells. After 9-10 days of differentiation, the M2 macrophages are cryopreserved using Cryo-SFM. Each cryo vial contains more than 5 million viable cells after thawing.

警告

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

例行更新培養

Click here for more information.

其他說明

Recommended Plating Density: 200000 cells per cm2Tested Markers: CD163 positive, CD68 positiveNote: The plated cells do not proliferate. Recommended plating density for obtaining an adherent cell monolayer with 70-90% confluency.

推薦產品

Recommended Primary Cell Culture Media:Link
Recommended Primary Cell Culture Media:Link

免責聲明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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商品

Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.

Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.

Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.

Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.

实验方案

Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.

使用无血清和无异源细胞培养基在10天内将人外周血单个核细胞(PBMC)体外分化为M1或M2巨噬细胞。欢迎探索我们超350种PromoCell产品。

Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.

Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.

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