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Merck

A8312

Sigma-Aldrich

m-氨基苯基硼酸-琼脂糖

aqueous suspension

别名:

(3-aminophenyl)boronic acid-Agarose, 3-Aminophenylboronic acid–Agarose, m-APBA-Agarose, m-Aminophenylboronic acid resin

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About This Item

MDL號碼:
分類程式碼代碼:
12161501
PubChem物質ID:
NACRES:
NA.32

形狀

aqueous suspension

品質等級

標籤範圍

40-80 μmol per mL

基質

6% beaded agarose

基質活化

epichlorohydrin

基質結合

through amino to carboxyls of EDTA

基質墊片

9 atoms

容量

≥8 mg/mL binding capacity (peroxidase Type VI)

儲存溫度

2-8°C

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相关类别

一般說明

固定在琼脂糖凝胶上的m-氨基苯硼酸(m-APBA)可用于捕获免疫球蛋白,但也适用于非特异性作用分子。m-APBA是一种硼酸亲和基质,主要用于高效亲和纯化单克隆抗体。

應用

m-氨基苯硼酸琼脂糖已用于:
  • 作为色谱柱成分,用于纯化大肠杆菌TOP10F细胞
  • 与各种细胞的裂解液混合,用于检测聚ADP核糖基化(PARylated)蛋白。
  • 辣根过氧化物酶(HRPO)的亲和纯化
  • 糖化人血清白蛋白(gHSA)纯化

外觀

悬浮于含0.002%醋酸氯己定的水中。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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T A Myöhänen et al.
The Biochemical journal, 197(3), 683-688 (1981-09-01)
The synthesis of 3-nitro-4-(6-aminohexylamido)phenylboronic acid is described. The properties of two novel forms of immobilized phenylboronate agarose adsorbents [m-aminophenylboronic acid-Matrex Gel and 3-nitro-4-(6-aminohexylamido)phenylboronic acid-Sepharose CL-6B] were investigated. Both gels bind and selectively retard the glycoprotein alpha-glucosidase from yeast. The retardation
RUNX poly (ADP-ribosyl) ation and BLM interaction facilitate the Fanconi anemia pathway of DNA repair
Tay L S, et al.
Testing, 24(7), 1747-1755 (2018)
Construction and optimization of a CC49-Based scFv-beta-lactamase fusion protein for ADEPT
Roberge M, et al.
Protein engineering, design & selection : PEDS, 19(4), 141-145 (2006)
Monika Kijewska et al.
Molecules (Basel, Switzerland), 25(3) (2020-02-14)
We report herein a novel ChemMatrix® Rink resin functionalised with two phenylboronate (PhB) moieties linked on the N-α and N-ε amino functions of a lysine residue to specifically capture deoxyfructosylated peptides, compared to differently glycosylated peptides in complex mixtures. The
Pravin K Bhatnagar et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 863(2), 235-241 (2008-02-09)
Hybrid hybridomas (quadromas) are derived by fusing at least two hybridomas, each producing a different antibody of predefined specificity. The resulting cell secretes not only the immunoglobulins of both parents but also hybrid molecules manifesting the binding characteristics of the

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