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Merck

A6017

Sigma-Aldrich

ω-Aminohexyl–Agarose

saline suspension

别名:

Omega-Aminohexyl-Agarose, 1,6-Diaminohexane–Agarose (N-linked), 6-Aminohexyl–Agarose

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About This Item

CAS号:
MDL编号:
UNSPSC代码:
23151817
NACRES:
NA.56

生物来源

plant

质量水平

表单

saline suspension

标记范围

≥5 μmol per mL

技术

affinity chromatography: suitable

基质

cross-linked 4% beaded agarose

基质活化

cyanogen bromide

基质附着

amino of 1,6-diaminohexane

基质隔离区

1 atom

容量

≥5 mg/mL binding capacity (bovine serum albumin)

适用性

suitable for chromatography

储存温度

2-8°C

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相关类别

一般描述

Hydrophobic ligands can serve as potentselective adsorbents. Both ω-aminoalkyl and alkyl agaroses can interact with the hydrophobic regions found in various proteins. The ω-aminoalkyl agarose product offered here consists of activated agarose with 1,6-diaminohexane covalently attached to one of its amine groups.

应用

ω-Aminohexyl–Agarose has been used in the conjugation of fatty acids with chain length from two carbons to 20 for fatty acyl beads pull-down experiments.
ω-aminohexyl–agarose has been used for coupling of serotype b-specific carbohydrate antigen (SbAg) of Actinobacillus actinomycetemcomitans Y4 for isolation of anti-SbAg antibodies using protein chromatography.

生化/生理作用

ω-aminoalkyl-agaroses are used in chromatography. In these, the protein is retained by lipophilic association between the hydrocarbon side chains on the agarose and hydrophobic pockets in the protein.

外形

Suspension in 0.5 M NaCl containing preservative

其他说明

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

象形图

Corrosion

警示用语:

Danger

危险声明

危险分类

Eye Dam. 1 - Skin Irrit. 2

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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A H Bakker et al.
Protein engineering, 8(3), 293-300 (1995-03-01)
Tissue-type plasminogen activator (t-PA) is composed of structurally autonomous domains. From the N-terminus of t-PA, a finger-like domain (F), an epidermal growth factor-like domain (G), two kringle domains (K1 and K2) and a serine protease domain (P) can be discerned.
Maria Cristiane Martins de Souza et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 878(5-6), 557-566 (2010-01-19)
The omega-aminohexyl diamine immobilized as ligand on CNBr- and bisoxirane-activated agarose gel was evaluated for the purification of human immunoglobulin G (IgG) from serum and plasma by negative affinity chromatography. The effects of matrix activation, buffer system, and feedstream on
M Lyon et al.
The Biochemical journal, 199(2), 419-426 (1981-11-01)
The binding of bovine testicular hyaluronidase to AH-Sepharose (1,6-diaminohexane--Sepharose) gels substituted with (1) dermatan sulphate, (2) desulphated dermatan sulphate, (3) heparin and (4) de-N/O-sulphated, re-N-acetylated heparin was investigated. Hyaluronidase was found to bind to (1) and (3), but not (2)
Rapid isolation of microbial milk-clotting enzymes by N-acetyl (or N-isobutyryl)-pepstatin--Aminohexylagarose.
H Kobayashi et al.
Analytical biochemistry, 122(2), 308-312 (1982-05-15)
Isa S Duarte et al.
Artificial organs, 30(8), 606-614 (2006-08-17)
This work investigated the potential use of an alternative adsorbent to anti-immunoglobulin E (IgE)-agarose for IgE selective adsorption therapy. A screening of several commercially available adsorbents (Concanavalin A, Lens culinaris[Lc], d-tryptophan, poly-l-lysine, and aminohexyl immobilized on agarose) was done through

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