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Merck

A4062

Sigma-Aldrich

Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

别名:

Rabbit Anti-Goat IgG (whole molecule)–AP

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About This Item

MDL编号:
UNSPSC代码:
12352203
NACRES:
NA.46

生物来源

rabbit

偶联物

alkaline phosphatase conjugate

抗体形式

affinity isolated antibody

抗体产品类型

secondary antibodies

克隆

polyclonal

表单

buffered aqueous glycerol solution

种属反应性

goat

技术

direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000

运输

wet ice

储存温度

2-8°C

靶向翻译后修饰

unmodified

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一般描述

Immunoglobulins (Igs) belongs immunoglobulin super-family. There are five different types of Immunoglobulins. Each immunoglobin have two heavy (H) and two light (L), held together by disulphide linkages. Each light comprises of one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region.

免疫原

purified goat IgG

应用

Alkaline phosphatase conjugated rabbit anti goat IgG was used as the secondary for ELISA assays to detect the binding of bacterial proteins to C components. The antibody was used at 1:5000 dilutions and detected using P-nitrophenyl phosphate at 1mg/ml (Sigma) as the substrate.
Anti-Goat IgG (whole molecule)-alkaline phosphatase antibody can be used in western blotting and immunoblotting (diluted 1: 20,000) .

生化/生理作用

IgG antibodies regulate several functions such as complement activation and phagocytosis. Thus, they play a crucial role in facilitating cytological immune responses. Anti-goat IgG (whole molecule)-alkaline phosphatase antibody (diluted 1: 5000) can be used for anti-OVA IgG detection. Rabbit anti- goat IgG (whole molecule)-alkaline phosphatase antibody reacts specifically with all goat immunoglobulins.

其他说明

Antibody adsorbed with human serum proteins.

外形

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 10 mM glycine, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide

制备说明

Adsorbed to reduce background staining with human samples.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Mario Pausa et al.
Journal of immunology (Baltimore, Md. : 1950), 170(6), 3214-3222 (2003-03-11)
Borrelia burgdorferi, the etiological agent of Lyme disease, comprises three genospecies, Borrelia garinii, afzelii, and burgdorferi sensu strictu, that exhibit different pathogenicity and differ in the susceptibility to C-mediated killing. We examined C-sensitive and C-resistant strains of B. burgdorferi for
Lizath M Aguiniga et al.
Infection and immunity, 84(11), 3131-3140 (2016-08-17)
Uropathogenic Escherichia coli (UPEC) accounts for 80 to 90% of urinary tract infections (UTI), and the increasing rate of antibiotic resistance among UPEC isolates reinforces the need for vaccines to prevent UTIs and recurrent infections. Previous studies have shown that
Ronja Mathiesen et al.
Acta veterinaria Scandinavica, 60(1), 36-36 (2018-06-20)
The significance of maternal immunoglobulin G (IgG) for the resistance against a number of infections affecting the health of young mink offspring is not known. Here, we present a validated immunoassay for quantification of mink IgG in serum and milk
Leila Choe et al.
Proteomics, 7(20), 3651-3660 (2007-09-21)
An 8-plex version of an isobaric reagent for the quantitation of proteins using shotgun methods is presented. The 8-plex version of the reagent relies on amine-labeling chemistry of peptides similar to 4-plex reagents. MS/MS reporter ions at 113, 114, 115
Nikita Deshpande et al.
Genetics, 209(4), 1085-1097 (2018-06-21)
Many heterogametic organisms adjust sex chromosome expression to accommodate differences in gene dosage. This requires selective recruitment of regulatory factors to the modulated chromosome. How these factors are localized to a chromosome with requisite accuracy is poorly understood. Drosophila melanogaster

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