79454

Ampliflu Red Westernblot Kit

for fluorescent detection

• UNSPSC代码: 12352200

属性

• 用途: sufficient for 50 applications
• 荧光: λ_ex 571 nm; λ_em 585 nm
• 储存温度: 2-8°C

说明

一般描述

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应用

The Ampliflu Red Western Blot Kit provides an alternative to the classical chemiluminescence detection. When working with Ampliflu Red, standard protocols for the immunoblotting-procedure can be used until the detection step. No extra time for signal accumulation is necessary as it is for chemiluminescent signals. The use of Ampliflu Red is suitable for protein quantities between 1 ng and 1 μg per band of protein.

原理

The kit contains the peroxidase substrate Ampliflu Red that is enzymatically converted to the highly fluorescent compound, resorufin. Fluorescent imaging is then performed on the immunoblot.

分析说明

Sensitivity: Limit of detection is 1 ng/band, but may vary depending on the antibodies used.

法律信息

Ampliflu is a trademark of Sigma-Aldrich Co. LLC

仅试剂盒组分

• Dimethyl sulfoxide, puriss. p.a., ACS reagent, ≥99.9% (GC) 3 mL

试剂盒组分也可单独购买

• 90101 Ampliflu^™ Red, for fluorescence, ≥98.0% (HPLC) 5 vial(s)
• 79382 Phosphate buffered saline, tablet 5 tablets

安全信息

• 象形图: GHS07
• 警示用语：: Warning
• 危险声明: H302
• 预防措施声明: P264 - P270 - P301 + P312 - P501
• 危险分类: Acute Tox. 4 Oral
• 储存分类代码: 10 - Combustible liquids
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable